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M9943

Sigma-Aldrich

10X MTP Taq Buffer

10X buffer, free of DNA contaminants; use with MTP Taq DNA Polymerase

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About This Item

UNSPSC Code:
41106306
NACRES:
NA.52

Quality Level

form

liquid

color

colorless

shipped in

wet ice

storage temp.

−20°C

General description

DNA contaminants can be introduced into PCR through a number of reagents. To minimize the risk of contaminant DNA during PCR, we offer 10x MTP Taq buffer to be used with MTP Taq DNA Polymerase (Product No. D7442). Each lot of MTP Taq buffer undergoes strict quality control testing to ensure the absence of contaminating DNA. To prevent false positive PCR results, only DNA-free reagents should be used in PCR reactions with MTP Taq DNA polymerase.

Application

10X MTPTaq Buffer has been used:

  • for the amplification of bacterial 16s rRNA genes from purified DNA
  • to amplify archaeal 16s rRNA
  • along with MTP Taq DNA polymerase (D7442) to amplify specific bacterial 16s rRNA genes from purified DNA

Legal Information

MTP is a trademark of Sigma-Aldrich Co. LLC

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
Florie Maillard et al.
PloS one, 14(4), e0214660-e0214660 (2019-04-10)
Increased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in
Florie Maillard et al.
Cells, 8(1) (2019-01-13)
Crohn's disease is characterized by abnormal ileal colonization by adherent-invasive E. coli (AIEC) and expansion of mesenteric adipose tissue. This study assessed the preventive effect of spontaneous physical activity (PA) on the gut-adipose tissue in a mouse model that mimics
Florie Maillard et al.
PloS one, 14(4), e0214660-e0214660 (2019-04-10)
Increased visceral adipose tissue and dysbiosis in the overweight and obese promote chronic inflammation. The aim of this study was to compare the effects of moderate-intensity continuous training (MICT) and high-intensity interval training (HIIT) on the gut-adipose tissue cross-talk in
Camille Mazière et al.
Microbiological research, 252, 126854-126854 (2021-08-29)
In order to understand the effect of human practices on microbial mats organisation, the study aimed to investigate the biodiversity within microbial mats from exploited and abandoned salterns. Despite several attempts, archaeal 16S rRNA gene fragment sequences were not obtained

Protocolos

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA.

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