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Merck

L7395

Sigma-Aldrich

Lipoxidase from Glycine max (soybean)

Type I-B, lyophilized powder, ≥50,000 units/mg solid

Sinónimos:

15-lipoxygenase, LOX A, Linoleate:oxygen oxidoreductase, Lipoxygenase

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type I-B

form

lyophilized powder

specific activity

≥50,000 units/mg solid

mol wt

94 kDa

shipped in

wet ice

storage temp.

−20°C

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General description

Lipoxidases (LOXs), or lipoxygenases, are nonheme iron proteins. Lipoxygenases are widely present in plants, some microorganisms, and animals. They are categorized as LOX-1, LOX-2, and LOX-3 with respect to pH and optimum temperature, substrate specificity, and level of inhibition by different agents.

Application

Lipoxidase from Glycine max (soybean) has been used:
  • to study the effects of 2-(α-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol(TMG) on oxidation of lipoproteins
  • as a representative enzyme for lipoxygenases in microplate assay platform to screen for enzymes degrading wood extractives
  • as a positive control in lipoxygenase inhibitory activity assay

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Biochem/physiol Actions

Lipoxidases are deactivated by heat treatment near 100°C for 5 to 10 minutes. Studies show that gamma irradiation treatment decreases the specific activity of soybean lipoxygenases. These enzymes are used in studies related to asthma and allergies. Lipoxidases are applicable for industrial bio-transformations such as in the production of aroma fine chemicals.
Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Unit Definition

One unit will cause an increase in A234 of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A234 unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Contains stabilizer and NaCl

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ewelina Zielińska et al.
Nutrients, 9(9) (2017-09-05)
This study investigated the effect of heat treatment of edible insects on antioxidant and anti-inflammatory activities of peptides obtained by in vitro gastrointestinal digestion and absorption process thereof. The antioxidant potential of edible insect hydrolysates was determined as free radical-scavenging
Chemical composition and lipoxygenase activity in soybeans (Glycine max L. Merr.) submitted to gamma irradiation
de Barros EA, et al.
Radiation Physics and Chemistry, 98, 29-32 (2014)
Microplate enzyme assay for screening lipoxygenases to degrade wood extractives
Nguyen D, et al.
Biocatalysis and Biotransformation, 25(2-4), 202-210 (2007)
Xiao-Hui Ma et al.
Signal transduction and targeted therapy, 7(1), 288-288 (2022-08-16)
Myocardial ischemia/reperfusion (I/R) injury is a classic type of cardiovascular disease characterized by injury to cardiomyocytes leading to various forms of cell death. It is believed that irreversible myocardial damage resulted from I/R occurs due to oxidative stress evoked during
Sereyvath Yoeun et al.
Frontiers in chemistry, 6, 500-500 (2018-11-15)
Allene oxide cyclase (AOC) is a key enzyme in the jasmonic acid (JA) biosynthetic pathway in plants, during which it catalyzes stereospecific conversion of 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid (12,13-EOT) to cis(+)-12-oxophytodienoic acid. Here, rice allene oxide cyclase (OsAOC) was localized to the

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