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L7386

Sigma-Aldrich

Lisostafina from Staphylococcus staphylolyticus

≥500 units/mg protein, lyophilized powder

Sinónimos:

Endopeptidasa glicil-glicina

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About This Item

Número de CAS:
9011-93-2
Comisión internacional de enzimas:
3.4.24.75 (BRENDA, IUBMB)
MDL number:
MFCD00131556
UNSPSC Code:
12352204
NACRES:
NA.54

product name

Lisostafina from Staphylococcus staphylolyticus, lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

biological source

microbial (Staphylococcus staphylotyticus)

Quality Level

300

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

25 kDa

composition

Protein, 50-70% biuret

packaging

pkg of (sold on basis of mg protein)

solubility

water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow

antibiotic activity spectrum

Gram-positive bacteria

application(s)

diagnostic assay manufacturing
diagnostic assay manufacturing

mode of action

cell wall synthesis | interferes

shipped in

wet ice

storage temp.

−20°C

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General description

Estructura química: péptido

Biochem/physiol Actions

La lisostafina es una endopeptidasa de cinc con un peso molecular de aproximadamente 25 kDa. Debido a que la lisostafina rompe los enlaces transversales de la poliglicina de la capa de peptidoglucanos de la pared celular de las especies de Staphylococcus , se ha encontrado útil para la lisis celular y también como potencial tratamiento antimicrobiano.
pH de actividad óptimo: ~7,5

Unit Definition

Una unidad reducirá la turbidez (A620) de una suspensión de células de Staphylococcus aureus de 0,250 a 0,125 en 10 min a un pH 7,5 a 37 °C en 6,0 ml de mezcla de reacción.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ian R Monk et al.
Nature communications, 10(1), 3067-3067 (2019-07-13)
WalKR (YycFG) is the only essential two-component regulator in the human pathogen Staphylococcus aureus. WalKR regulates peptidoglycan synthesis, but this function alone does not explain its essentiality. Here, to further understand WalKR function, we investigate a suppressor mutant that arose
Catherine Bennett et al.
International forum of allergy & rhinology, 9(8), 918-925 (2019-06-05)
Chronic rhinosinusitis (CRS) is a common disease, often refractory to conventional antimicrobial treatment. In this study we investigate the antimicrobial and anti-inflammatory effects of adding kappa-carrageenan to a commercially available sinus rinse. Kappa-carrageenan was added to Flo CRS and Flo
Judy J J Ou et al.
Frontiers in cellular and infection microbiology, 6, 187-187 (2017-01-14)
Background:Staphylococcus aureus (S. aureus) small colony variants (SCVs) can survive within the host intracellular milieu and are associated with chronic relapsing infections. However, it is unknown whether host invasion rates and immune responses differ between SCVs and their wild-type counterparts.
Dafne Bongiorno et al.
MicrobiologyOpen, 9(5), e1017-e1017 (2020-04-03)
Methicillin-resistant S. aureus has been reported as the main pathogen involved in chronic infections, osteomyelitis, and prosthetic joint infections. The host/pathogen interaction is dynamic and requires several changes to promote bacterial survival. Here, we focused on the internalization and persistence
Mathias Schmelcher et al.
Applied and environmental microbiology, 78(7), 2297-2305 (2012-01-31)
Staphylococci cause bovine mastitis, with Staphylococcus aureus being responsible for the majority of the mastitis-based losses to the dairy industry (up to $2 billion/annum). Treatment is primarily with antibiotics, which are often ineffective and potentially contribute to resistance development. Bacteriophage
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