HCT-8

90032006, human intestine, Epithelial

• UNSPSC Code: 41106514

Propiedades

• product name: HCT-8, 90032006
• biological source: human intestine
• growth mode: Adherent
• karyotype: 2n = 46, pseudodiploid
• morphology: Epithelial
• products: Carcinoembryonic antigen (CEA)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Cell Line Origin

Human ileocecal adenocarcinoma

Cell Line Description

From a 67 year old male. The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin.

Application

Tumourigenicity studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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