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C9658

Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Sinónimos:

γ-Glutamyl hydrolase

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About This Item

Número de CAS:
9074-87-7
Comisión internacional de enzimas:
3.4.17.11 (BRENDA, IUBMB)
EC Number:
232-978-1
MDL number:
MFCD00130719
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

300

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H2O: soluble 0.5 mg/mL

storage temp.

−20°C

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Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified
Solutions should be prepared fresh prior to use.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Klára Hlouchová et al.
Journal of neurochemistry, 101(3), 682-696 (2007-01-24)
Human glutamate carboxypeptidase II (GCPII) is a transmembrane metallopeptidase found mainly in the brain, small intestine, and prostate. In the brain, it cleaves N-acetyl-L-aspartyl-glutamate, liberating free glutamate. Inhibition of GCPII has been shown to be neuroprotective in models of stroke
S G Sligar et al.
Proceedings of the National Academy of Sciences of the United States of America, 71(10), 3906-3910 (1974-10-01)
Methylene hydroxylation by cytochrome P-450(cam) (cytochrome m) can be resolved into four distinct steps: substrate addition, m(o) --> m(os); reduction, m(os) --> m(rs); dioxygen addition, m(rs) --> m(O2) (rs); followed by a second putidaredoxin (Pseudomonas putida ferredoxin)-mediated reduction and product
Luz I A Calderon-Villalobos et al.
The Plant cell, 17(9), 2473-2485 (2005-07-19)
In this study, we characterize the evolutionarily conserved TOUGH (TGH) protein as a novel regulator required for Arabidopsis thaliana development. We initially identified TGH as a yeast two-hybrid system interactor of the transcription initiation factor TATA-box binding protein 2. TGH
Valentina Carozzi et al.
CNS & neurological disorders drug targets, 7(4), 348-360 (2008-11-11)
The role of Glutamate (Glu), one of the major excitatory neurotransmitters in the central nervous system, has been thoroughly investigated in animal models and in humans in several physiologic events, such as brain development and synaptic plasticity, but also in
Hiroki Nagai et al.
International journal of oncology, 41(1), 24-30 (2012-05-04)
Pemetrexed (PEM) is a novel, multitargeted, antifolate, antineoplastic agent for the treatment of non-small cell lung cancer and malignant pleural mesothelioma. Additional effects of nitric oxide (NO) donors on the chemosensitivity of cancers have been reported. However, the effects of
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