A6043
Albumin, biotinamidocaproyl labeled bovine
lyophilized powder
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About This Item
Productos recomendados
biological source
bovine
Quality Level
assay
≥80% protein basis (biuret)
form
lyophilized powder
origin
USA origin
extent of labeling
≥8 mol biotin per mol albumin
technique(s)
bioconjugation: suitable
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
Albumin-biotin conjugate was used to develop a sensitive non-isotopic benzodiazepine receptor assay. The method can be generalized to other receptors.
Physical form
Lyophilized powder containing sodium citrate buffer salts
Preparation Note
Prepared from albumin (A7638)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Hiroshi Sekiguchi et al.
Ultramicroscopy, 109(8), 916-922 (2009-04-07)
We have developed a method to detect specific proteins with a high sensitivity using a gel electrophoresis method and force measurement of atomic force microscopy (AFM). Biotinylated proteins were separated by electrophoresis and fixed with cross-linking chemicals on the gel
S M Costello et al.
Clinical chemistry, 25(9), 1572-1580 (1979-09-01)
Classically, one can increase the titer of an agglutinating or first antibody with an antiglobulin or second antibody. We have used an avidin-biotin system in place of antiglobulin to similarly extend the agglutination by an initial anticellular antibody. Erythrocytes were
Akane Furuta et al.
The Journal of biological chemistry, 284(9), 5927-5935 (2009-01-07)
Outer arm dynein (OAD) of cilia and flagella contains two or three distinct heavy chains, each having a motor function. To elucidate their functional difference, we compared the in vitro motile properties of Chlamydomonas wild-type OAD containing the alpha, beta
Keitaro Shibata et al.
PloS one, 7(8), e42990-e42990 (2012-08-18)
Cytoplasmic dynein and kinesin are two-headed microtubule motor proteins that move in opposite directions on microtubules. It is known that kinesin steps by a 'hand-over-hand' mechanism, but it is unclear by which mechanism dynein steps. Because dynein has a completely
Jens Friedrichs et al.
Nature protocols, 5(7), 1353-1361 (2010-07-03)
Atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) enables the quantitative study of cell adhesion under physiological conditions. SCFS probes adhesive interactions of single living cells with substrates such as extracellular matrix (ECM) proteins and other cells. Here we present
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