The product is not tested for microbial contamination. Instead, a fluorometric toxicity assay is conducted. This involves growing three mammalian cell lines in the presence of the test product. After 7 days of incubation, the fluorescence is measured, and it is directly proportional to cell density.
A4034
(+)-Sodium L-ascorbate
powder, BioReagent, suitable for cell culture
Sinónimos:
L(+)-Ascorbic acid sodium salt, Vitamin C sodium salt
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Fórmula empírica (notación de Hill):
C6H7NaO6
Número CAS:
Peso molecular:
198.11
NACRES:
NA.75
PubChem Substance ID:
eCl@ss:
34058010
UNSPSC Code:
12352205
EC Number:
205-126-1
MDL number:
Beilstein/REAXYS Number:
3767246
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Servicio técnico
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Permítanos ayudarlebiological source
synthetic (organic)
Quality Segment
product line
BioReagent
assay
≥98%
form
powder
mol wt
Mw 198.11 g/mol
technique(s)
cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
mp
220 °C (dec.) (lit.)
solubility
H2O: 50 mg/mL
SMILES string
[Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-]
InChI
1S/C6H8O6.Na/c7-1-2(8)5-3(9)4(10)6(11)12-5;/h2,5,7-10H,1H2;/q;+1/p-1/t2-,5+;/m0./s1
InChI key
PPASLZSBLFJQEF-RXSVEWSESA-M
General description
(+)-Sodium L-ascorbate (SA) is a potential antioxidant and is a derivative of vitamin C (L-ascorbic acid).[1] It is widely used as a food additive. In addition, SA enhances growth retardation activity of bleomycin sulfate, R020-1724 (an inhibitor of cyclic nucleotide phosphodiesterase), prostaglandin E1 and sodium butyrate agents on neuroblastoma cells in culture. Elevated levels of SA induces two-stage urinary bladder carcinogenesis in rats. sodium L-ascorbate in combination with collagenogenesis inhibitors is used to treat dermal scars and keloids.[1]
Application
(+)-Sodium L-ascorbate has been used:
- to produce H2O2 in cell culture media
- as a reducing agent for a cell-permeable artificial electron donor, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD)
- to study its effect on production of collagen and elastic fibers in fibroblasts cultures derived from normal human skin and dermal fat[1]
Used for preparation of plant extracts; reductant for the isolation of chloroplasts.
Biochem/physiol Actions
Antioxidant
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Este artículo | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable, cell culture | plant: suitable | technique(s) - | technique(s) HPLC: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
| biological source synthetic (organic) | biological source - | biological source synthetic | biological source - |
| assay ≥98% | assay ≥99.0% (NT) | assay ≥98% | assay - |
| form powder | form powder or crystals | form crystalline | form - |
| solubility H2O: 50 mg/mL | solubility water: 642.6 g/L at 20 °C | solubility - | solubility - |
| color white to off-white | color white | color white to off-white | color - |
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Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 1
ppe
Eyeshields, Gloves, type N95 (US)
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| A4034-1KG | 04061833368428 |
| A4034-100G | 04061833368411 |
| A4034-500G | 04061833368435 |
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How was the sodium L-ascorbate product A4034 validated for cell culture applications? A customer is concerned about its sterility and wants to confirm that it will not harm cells in medium and needs more information on the validation process.
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