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Documentos

03-3734

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

Sinónimos:

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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About This Item

Fórmula empírica (notación de Hill):
C14H15BrClNO6
Número de CAS:
7240-90-6
Peso molecular:
408.63
Beilstein/REAXYS Number:
1402009
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
329747611

availability

available only in Japan

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

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Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Suitability

for biological purposes

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Jennifer R Dwyer et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(37), E2486-E2495 (2012-08-22)
The three lipin phosphatidate phosphatase (PAP) enzymes catalyze a step in glycerolipid biosynthesis, the conversion of phosphatidate to diacylglycerol. Lipin-1 is critical for lipid synthesis and homeostasis in adipose tissue, liver, muscle, and peripheral nerves. Little is known about the
Hiroyuki Kanazawa et al.
PloS one, 6(4), e19195-e19195 (2011-05-12)
Ischemia-reperfusion (I/R) injury associated with living donor liver transplantation impairs liver graft regeneration. Mesenchymal stem cells (MSCs) are potential cell therapeutic targets for liver disease. In this study, we demonstrate the impact of MSCs against hepatic I/R injury and hepatectomy.
Yibing Wang et al.
PloS one, 8(3), e59195-e59195 (2013-03-26)
Our study had three objectives: to extend the plasmid-based transformation protocol to a clinical isolate of C. trachomatis belonging to the trachoma biovar, to provide "proof of principle" that it is possible to "knock out" selected plasmid genes (retaining a
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