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Key Documents

MAB3494

Sigma-Aldrich

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

clone 4.3E8.D1, Chemicon®, from mouse

Sinónimos:

Mitochondrial Marker

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4.3E8.D1, monoclonal

species reactivity

mouse, rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATP5B(506)

Specificity

Recognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria.

Immunogen

Epitope: beta chain
Rat brain mitochondria

Application

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP.
Immunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Alyssa Torres et al.
Frontiers in immunology, 14, 1103231-1103231 (2023-08-02)
Glucose metabolism, specifically, hexokinase 2 (HK2), has a critical role in rheumatoid arthritis (RA) fibroblast-like synoviocyte (FLS) phenotype. HK2 localizes not only in the cytosol but also in the mitochondria, where it protects mitochondria against stress. We hypothesize that mitochondria-bound
Daan M Van Den Brink et al.
PLoS genetics, 14(9), e1007627-e1007627 (2018-09-11)
Increasing evidence suggests that dysregulation of lipid metabolism is associated with neurodegeneration in retinal diseases such as age-related macular degeneration and in brain disorders such as Alzheimer's and Parkinson's diseases. Lipid storage organelles (lipid droplets, LDs), accumulate in many cell
Gowtham K Annarapu et al.
Redox biology, 48, 102205-102205 (2021-12-11)
Hemolysis, a pathological component of many diseases, is associated with thrombosis and vascular dysfunction. Hemolytic products, including cell-free hemoglobin and free heme directly activate platelets. However, the effect of hemolysis on platelet degranulation, a central process in not only thrombosis
Pamela J Yao et al.
Molecular biology of the cell, 28(3), 387-395 (2016-12-10)
Mitochondria are essential organelles whose biogenesis, structure, and function are regulated by many signaling pathways. We present evidence that, in hippocampal neurons, activation of the Sonic hedgehog (Shh) signaling pathway affects multiple aspects of mitochondria. Mitochondrial mass was increased significantly
Hye-Won Hyun et al.
Frontiers in cellular neuroscience, 10, 99-99 (2016-05-06)
High mobility group box 1 (HMGB1) acts a signaling molecule regulating a wide range of inflammatory responses in extracellular space. HMGB1 also stabilizes nucleosomal structure and facilitates gene transcription. Under pathophysiological conditions, nuclear HMGB1 is immediately transported to the cytoplasm

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