393207-M

Anti-HNE-Michael Adducts, Reduced Rabbit pAb

liquid, Calbiochem^®

• Sinónimos: Anti-4-Hydroxy-2-noneal Michael Adducts, Reduced
• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: serum
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• Formulario: liquid
• no contiene: preservative
• reactividad de especies (predicha por homología): all
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• isotipo: IgG
• temp. de almacenamiento: −70°C
• modificación del objetivo postraduccional: unmodified

Descripción

Descripción general

Anti-HNE-Michael Adducts, Reduced, rabbit polyclonal, specifically recognizes chemically reduced amino acid-(4)-HNE adducts. It is validated for use in ELISA, WB & IHC (frozen & paraffin sections).

Rabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.

Recognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.

The CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer′s disease.

Especificidad

A broad range of species

Inmunógeno

HNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced

Aplicación

ELISA (1:500-1:1000)

Immunoblotting (1:1000-1:4000)

Frozen Sections (1:50-1:500)

Paraffin Sections (1:50-1:500)

Advertencia

Toxicity: Standard Handling (A)

Forma física

Serum diluted in 50% glycerol, pH 7.5.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Does not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.

Toyokuni, S., et al. 2000. Antioxid. Redox. Signal2, 681.
Neely, M.D., et al. 1999. J. Neurochem.72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun.212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys.324, 241.
Uchida, K., et al. 1994. Biochemistry33, 12487.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1