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  • [Inquiry of antibodies anti-Neospora caninum in dairy cattle, dogs and rural workers of the south-west region of Mato Grosso State].

[Inquiry of antibodies anti-Neospora caninum in dairy cattle, dogs and rural workers of the south-west region of Mato Grosso State].

Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria (2010-05-18)
Ana Helena Benetti, Fábio Bernardo Schein, Thaís Rabelo dos Santos, Gilson Hélio Toniollo, Alvimar José da Costa, José Roberto Mineo, Janaína Lobato, Deise Aparecida de Oliveira Silva, Solange Maria Gennari
ABSTRACT

Considering the importance of neosporosis in the animal health and production, the frequency of antibodies to Neospora caninum was evaluated in dairy cattle of the Southwestern region of Mato Grosso State, Brazil, in addition to serum samples obtained from dogs and humans living in the farms. A total of 1036 serum samples were analyzed, from which 932 were from dairy bovine females, 37 from dogs and 67 from humans, from 24 farms and examined by the indirect fluorescent antibody test (IFAT). Reactive human serum samples were retested by Western- blotting to confirm the results. Antibodies to N. caninum were found in 499 cattle sera (53.5%), with at least one positive in each farm, 25 dog sera (67.6%) and seven human sera (10.5%). There was no significant difference in the number of positive cattle sera according to age group. The results indicate a wide dissemination of N. caninum in the studied region.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Bovine IgG (whole molecule)–FITC antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG (γ-chain specific)−FITC antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Human IgG (γ-chain specific)−Peroxidase antibody produced in goat, affinity isolated antibody, buffered aqueous solution