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F9887

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Rabbit IgG (whole molecule)–Fluorescein isothiocyanate

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About This Item

MDL number:
MFCD00162786
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

contains

≥15 mM sodium azide as preservative

storage condition

protect from light

technique(s)

direct immunofluorescence: 1:160
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
There are four classes of IgG namely IgG1, IgG2, IgG3, and IgG4. Among them IgG1 is the most abundant antibody.

Specificity

Binds all rabbit Igs.

Immunogen

Purified rabbit IgG

Application

Anti-Rabbit IgG (whole molecule)-FITC antibody produced in goat has been used in immunohistochemistry and indirect immunofluorescence.

Biochem/physiol Actions

Immunoglobulin G (IgG) provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgG1 deficiency results in hypogammaglobulinemia. IgG2 deficiency increases susceptibility to bacterial infections IgG3 mediates effector functions IgG4 are associated with asymptomatic infection. IgG antibody have enormous therapeutic potential and the Fc region is involved for the development of therapeutic antibody.

Other Notes

Antibody adsorbed with human IgG.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, with 15 mM sodium azide as a preservative.

Preparation Note

Adsorbed to reduce background staining with human samples.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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