HPLC Analysis of Norphenylephrine Enantiomers on Astec^® CYCLOBOND I 2000 AC (pH 4.1)

column

CYCLOBOND I 2000 AC, 25 cm x 4.6 mm I.D., 5 μm particles (20124AST)

mobile phase

[A] 20 mM ammonium acetate, pH 4.1; [B] methanol; (93:7, A:B)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 254 nm

injection

1 μL

sample

1 mg/mL in methanol