Saltar al contenido
Merck

Preparation of asparagine-linked monoglucosylated high-mannose-type oligosaccharide from egg yolk.

Carbohydrate research (2015-05-15)
Ning Wang, Akira Seko, Yoichi Takeda, Yukishige Ito
RESUMEN

Monoglucosylated high-mannose-type glycan (Glc1Man9GlcNAc2: G1M9) is well-known as a key glycoform in the glycoprotein folding process, which is specifically recognized by lectin chaperones calnexin (CNX) and calreticulin (CRT) in the endoplasmic reticulum (ER). In this work, we developed an efficient method for the preparation of G1M9-Asn. The G1M9-Asn was obtained from the IgY-rich fraction derived from hen egg yolk by the digestion with pronase. The α-amino group of asparagine in G1M9-Asn was protected with the 9-fluorenylmethyloxycarbonyl (Fmoc) group and the labeled glycans were subsequently purified using high performance liquid chromatography (HPLC). This method will provide useful substrates for analysis of the glycoprotein folding cycle in the ER.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Cloruro de sodio, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Cloruro de sodio, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
SAFC
Sodium chloride solution, 5 M
Sigma-Aldrich
Metanol, anhydrous, 99.8%
Sigma-Aldrich
Cloruro de sodio, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
Metanol, JIS special grade, ≥99.8%
Sigma-Aldrich
2,5-Dihydroxybenzoic acid, 98%
Sigma-Aldrich
Cloruro de sodio, JIS special grade, ≥99.5%
Sigma-Aldrich
Sodium chloride solution, BioUltra, for molecular biology, ~5 M in H2O
Sigma-Aldrich
Cloruro de sodio, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
Cloruro de sodio, 99.999% trace metals basis
Supelco
2,5-Dihydroxybenzoic acid, matrix substance for MALDI-MS, >99.0% (HPLC)
Sigma-Aldrich
Sodium chloride solution, 5 M
Sigma-Aldrich
Metanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Cloruro de sodio, SAJ first grade, ≥99.0%
Sigma-Aldrich
Fmoc N-hydroxysuccinimide ester, ≥98.0% (HPLC)
Sigma-Aldrich
Cloruro de sodio, meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%
Sigma-Aldrich
Cloruro de sodio, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
Metanol, SAJ special grade
Sigma-Aldrich
Metanol, suitable for HPLC
Sigma-Aldrich
Cloruro de sodio, AnhydroBeads, −10 mesh, 99.999% trace metals basis
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Sodium chloride solution, 0.85%
Sigma-Aldrich
Sodium chloride solution, 1 M
Sigma-Aldrich
Cloruro de sodio, random crystals, optical grade, 99.9% trace metals basis
Sigma-Aldrich
Metanol, suitable for HPLC, gradient grade, 99.93%
Sigma-Aldrich
Metanol, NMR reference standard
Sigma-Aldrich
Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.