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  • Analyte induced water adsorbability in gas phase biosensors: the influence of ethinylestradiol on the water binding protein capacity.

Analyte induced water adsorbability in gas phase biosensors: the influence of ethinylestradiol on the water binding protein capacity.

The Analyst (2015-03-13)
Borys Snopok, Ivanna Kruglenko
RESUMEN

An ultra-sensitive gas phase biosensor/tracer/bio-sniffer is an emerging technology platform designed to provide real-time information on air-borne analytes, or those in liquids, through classical headspace analysis. The desired bio-sniffer measures gaseous 17α- ethinylestradiol (ETED) as frequency changes on a quartz crystal microbalance (QCM), which is a result of the interactions of liquid sample components in the headspace (ETED and water) with a biorecognition layer. The latter was constructed by immobilization of polyclonal antiserum against a phenolic A-ring of estrogenic receptors through protein A. The QCM response exhibited stretched exponential kinetics of negative frequency shifts with reversible and "irreversible" components of mass uptake onto the sensor surface in static headspace conditions when exposed to water solutions of ETED over the sensor working range, from 10(-10) to 10(-17) g L(-1). It was shown that the variations in the QCM response characteristics are due to the change of the water-binding capacity of the sensing layer induced by protein transformations initiated by the binding of ETED molecules. This result is well correlated with the natural physiological function of estrogens in controlling the homeostasis of body fluids in living beings.

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Sigma-Aldrich
Ceftazidime hydrate, 90.0-105.0%, contains ~10% sodium carbonate as stabilizer
Sigma-Aldrich
17α-etinilestradiol, ≥98%
Sigma-Aldrich
Tiocianato de guanidina, for molecular biology, ≥99%
Sigma-Aldrich
Tiocianato de guanidina, BioReagent, for molecular biology, ≥99%
Sigma-Aldrich
Ceftazidime hydrate
USP
Etinil estradiol, United States Pharmacopeia (USP) Reference Standard
USP
Ceftazidime pentahydrate, United States Pharmacopeia (USP) Reference Standard
Supelco
17α-etinilestradiol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Tiocianato de guanidina, BioUltra, for molecular biology, ≥99.0% (AT)
Sigma-Aldrich
Tiocianato de guanidina, ≥97% (titration)
17α-etinilestradiol, European Pharmacopoeia (EP) Reference Standard
17α-etinilestradiol, British Pharmacopoeia (BP) Assay Standard
17α-etinilestradiol, European Pharmacopoeia (EP) Reference Standard