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Calorie restriction does not elicit a robust extension of replicative lifespan in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America (2014-07-30)
Daphne H E W Huberts, Javier González, Sung Sik Lee, Athanasios Litsios, Georg Hubmann, Ernst C Wit, Matthias Heinemann
RESUMEN

Calorie restriction (CR) is often described as the most robust manner to extend lifespan in a large variety of organisms. Hence, considerable research effort is directed toward understanding the mechanisms underlying CR, especially in the yeast Saccharomyces cerevisiae. However, the effect of CR on lifespan has never been systematically reviewed in this organism. Here, we performed a meta-analysis of replicative lifespan (RLS) data published in more than 40 different papers. Our analysis revealed that there is significant variation in the reported RLS data, which appears to be mainly due to the low number of cells analyzed per experiment. Furthermore, we found that the RLS measured at 2% (wt/vol) glucose in CR experiments is partly biased toward shorter lifespans compared with identical lifespan measurements from other studies. Excluding the 2% (wt/vol) glucose experiments from CR experiments, we determined that the average RLS of the yeast strains BY4741 and BY4742 is 25.9 buds at 2% (wt/vol) glucose and 30.2 buds under CR conditions. RLS measurements with a microfluidic dissection platform produced identical RLS data at 2% (wt/vol) glucose. However, CR conditions did not induce lifespan extension. As we excluded obvious methodological differences, such as temperature and medium, as causes, we conclude that subtle method-specific factors are crucial to induce lifespan extension under CR conditions in S. cerevisiae.

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Millipore
D-(+)-Glucosa monohydrate, suitable for microbiology, ≥99.0%
Sigma-Aldrich
D-(+)-Glucosa monohydrate, 97.5-102.0% anhydrous basis, meets USP testing specifications
Sigma-Aldrich
D-(+)-Glucosa monohydrate, meets analytical specification of Ph. Eur., BP, Ph Franç., 97.5-102.0% anhydrous basis(HPLC)
Sigma-Aldrich
D-(+)-Glucosa monohydrate, tested according to Ph. Eur.