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Merck

Streptavidin-biotin technology: improvements and innovations in chemical and biological applications.

Applied microbiology and biotechnology (2013-09-24)
Christopher M Dundas, Daniel Demonte, Sheldon Park
RESUMEN

Streptavidin and its homologs (together referred to as streptavidin) are widely used in molecular science owing to their highly selective and stable interaction with biotin. Other factors also contribute to the popularity of the streptavidin-biotin system, including the stability of the protein and various chemical and enzymatic biotinylation methods available for use with different experimental designs. The technology has enjoyed a renaissance of a sort in recent years, as new streptavidin variants are engineered to complement native proteins and novel methods of introducing selective biotinylation are developed for in vitro and in vivo applications. There have been notable developments in the areas of catalysis, cell biology, and proteomics in addition to continued applications in the more established areas of detection, labeling and drug delivery. This review summarizes recent advances in streptavidin engineering and new applications based on the streptavidin-biotin interaction.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Biotina, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
USP
Biotina, United States Pharmacopeia (USP) Reference Standard
Millipore
Estreptavidina, 5 mg Streptavidin for ELISA & Western Blotting.
SAFC
Biotina
Sigma-Aldrich
Biotina, ≥99.0% (T)
Supelco
Biotina, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
LiCl Immune Complex Wash Buffer, For use in Chromatin Immunoprecipitation assays.
Sigma-Aldrich
Biotina, meets USP testing specifications
Supelco
Biotina, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Biotina, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Biotina, tested according to Ph. Eur.
Sigma-Aldrich
Biotina, SAJ special grade, ≥98.0%