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Key Documents

59142C40

Supelco

SUPELCOSIL LC-ABZ (5 µm) HPLC Columns

L × I.D. 25 cm × 4 mm, HPLC Column

Sinónimos:

Analytical LC-ABZ Chromatography Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Columna de HPLC SUPELCOSIL LC-ABZ, 5 μm particle size, L × I.D. 25 cm × 4 mm

material

stainless steel column

agency

suitable for USP L60

product line

SUPELCOSIL

feature

endcapped

manufacturer/tradename

SUPELCOSIL

packaging

1 ea of

extent of labeling

12.0% carbon loading

parameter

≤70 °C temp. limit
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4 mm

surface area

170 m2/g

surface coverage

surface coverage 3.4 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

amide, alkyl phase

particle size

5 μm

pore size

120 Å

pH range

2-7.5

application(s)

food and beverages

separation technique

reversed phase

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General description

SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing additives and techniques such as competing amines, high ionic strength, and pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.

Application


  • Determination of active substances in binary mixture antiparasitic veterinary formulations by HPLC: This study showcases the use of the SUPELCOSIL LC-ABZ HPLC column for the precise determination of active compounds in veterinary medicine. This application is crucial in ensuring the effectiveness and safety of veterinary drugs. The study highlights the column′s capability to handle complex mixtures in pharmaceutical formulations, demonstrating its effectiveness in both academic research settings and in quality control for pharmaceutical applications (Kulik et al., 2011).

Features and Benefits

• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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D Korakas et al.
Journal of pharmaceutical and biomedical analysis, 12(9), 1141-1145 (1994-09-01)
A series of biologically active peptides and their conjugates with lipidic amino acids were investigated by systematic change of the mobile phase composition using traditional octadecylsilica stationary phase and the newly developed Supelcosil LC-ABZ column. The mobile phases contained various
Murali Pendela et al.
Journal of separation science, 32(20), 3418-3424 (2009-09-19)
Streptomycin (SM) is composed of streptidine, streptose and N-methyl glucosamine sugar moieties. For the determination of SM and its related substances, an ion-pair LC-UV detection method using a Supelcosil LC-ABZ column was developed previously. While analyzing commercial samples, several unknown
Q C Li et al.
Journal of chromatographic science, 38(6), 246-254 (2000-07-13)
Nevirapine (Viramune), a dipyridiodiazepinone, is a potent and highly specific nonnucleoside inhibitor of HIV-1 reverse transcriptase. This paper describes the validation of a specific, sensitive, and stability-indicating high-performance liquid chromatography method for the assay and determination of related organic impurities
J A López et al.
Journal of chromatography. A, 870(1-2), 105-114 (2000-03-18)
A high-performance liquid chromatographic method with electrochemical detection has been developed for the determination of six 1,4-dihydropyridines: nifedipine, nimodipine, nisoldipine, nicardipine, felodipine and lacidipine. The chromatographic separation was performed using a Supelcosil LC-ABZ+Plus C18 column. A mobile phase of methanol-water
Ana B Baranda et al.
Journal of chromatography. A, 1031(1-2), 275-280 (2004-04-03)
A high-performance liquid chromatographic method with electrochemical detection was developed for the simultaneous determination of five 1,4-dihydropyridines: amlodipine, nitrendipine, felodipine, lacidipine and lercanidipine. These drugs are widely used in the treatment of hypertension, angina pectoris and the therapy of cerebrovascular

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