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Key Documents

SRP6119

Sigma-Aldrich

DHFR from mouse

recombinant, expressed in E. coli, ≥95% (SDS-PAGE)

Sinónimos:

DYR, Dihydrofolate reductase, N/A

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

assay

≥95% (SDS-PAGE)

form

liquid

mol wt

23.8 kDa (207 aa, 1-187 aa + NT His-Tag)

packaging

pkg of 100 μg

NCBI accession no.

P00375

UniProt accession no.

P00375

shipped in

dry ice

storage temp.

−70°C

Gene Information

mouse ... DHFR(13361)

General description

Dihydrofolate reductase (DHFR) is a member of the reductase family of enzymes that is ubiquitously expressed in all organisms. The gene encoding this protein is localized on mouse chromosome 13.

Biochem/physiol Actions

DHFR catalyzes the nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reduction of dihydrofolate to tetrahydrofolate, and it is essential for the synthesis of thymidylate, purines and several amino acids. Expression of methotrexate (MTX)-resistant variants of DHFR in normal hematopoietic cells is a potential strategy to permit administration of larger doses of MTX by alleviating drug toxicity in normal cells and tissues that are drug sensitive.

Physical form

1 mg/mL solution in 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 2 mM DTT and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Other Notes

MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Dihydrofolate reductase gene variations in susceptibility to disease and treatment outcomes.
Askari BS and Krajinovic M
Current Genomics, 11(8), 578-583 (2010)
A molecular genetic linkage map of mouse chromosome 13 anchored by the beige (bg) and satin (sa) loci.
Justice MJ
Genomics, 6(2), 341-351 (1990)
M N Lombardo et al.
Drug metabolism and disposition: the biological fate of chemicals, 47(9), 995-1003 (2019-06-16)
Pharmacokinetic/pharmacodynamic properties are strongly correlated with the in vivo efficacy of antibiotics. Propargyl-linked antifolates, a novel class of antibiotics, demonstrate potent antibacterial activity against both Gram-positive and Gram-negative pathogenic bacteria, including multidrug-resistant Staphylococcus aureus Here, we report our efforts to

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