RAB0278

Rat IL-1 β ELISA Kit

for cell and tissue lysates

• Sinónimos: Il-1 beta, Interleukin-1 beta
• UNSPSC Code: 41116158
• NACRES: NA.32

Propiedades

• species reactivity: rat
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable; capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 80 pg/mL; standard curve range: 68.59-50000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... Il1b(24494)

Descripción

General description

The Rat IL-1 β ELSA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat IL-1β cell lysate and tissue lysate.

Immunogen

Recombinant Rat IL-1 β

Application

For research use only. Not for use in diagnostic procedures

Rat IL-1 Β ELISA Kit has been used to measure IL-1 Β levels .
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Pro-inflammatory cytokine IL-1 Β (IL1B), which is highly expressed on Β-adrenoreceptor in obese adipose tissues, is implicated in induction of uncoupling protein 1 (UCP1) expression via extracellular signal-regulated kinase (ERK) activation in adipocytes. Macrophage autophagy inhibits the production of inflammasome-dependent IL-1Β and thereby it plays a vital role in reducing acute toxin-induced liver injury and death. In rats, IL-1Β enhanced neurogenesis and plasticity of carotid bodies (CB) by triggering ERK1/2 activation. IL-1Β plays a vital role in alteration of glomerular hemodynamics via stimulating the production of prostaglandin E2 (PGE2) in the mesangial cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Los componentes del kit también están disponibles por separado

• RABDIL6 ELISA 5X Sample Diluent Buffer (Item D2)
• RABELADE ELISA 5X Assay/Sample Diluent Buffer E (Item E2)
• RABLYSIS1 2X Cell Lysis Buffer (Item J)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Información de seguridad

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 8A - Combustible corrosive hazardous materials