Saltar al contenido
Merck
Todas las fotos(1)

Key Documents

View All Documentation

L2769

Sigma-Aldrich

Lectina de Phaseolus vulgaris (frijol rojo)

Leucoagglutinin PHA-L, lyophilized powder

Sinónimos:

Aglutinina de Phaseolus vulgaris, PHA

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
Todas las fotos(1)

About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Phaseolus vulgaris

form

lyophilized powder

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.)
≤1.0 μg/mL leucoagglutination activity
≤5 μg/mL mitogenic activity

composition

Protein, ≥60% biuret

impurities

salt, free

storage temp.

2-8°C

¿Está buscando productos similares? Visita Guía de comparación de productos

General description

Phytohemagglutinin (PHA) derived from Phaseolus vulgaris is a tetrameric glycoprotein. It consists of two different subunits, that weighs 120 kDa.

Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • as a positive control in ELISPOT (enzyme-linked immunospot) assay
  • to induce Th2 polarization for T-cell differentiation in vitro
  • as controls in the lymphocyte proliferation assays

Biochem/physiol Actions

La PHA consta de dos especies moleculares, la eritroaglutinina (PHA-E), que tiene poca actividad mitógena y elevada actividad de eritroaglutinina, y la leoucoaglutinina (PHA-L), que tiene elevada actividad mitógena y leoucoaglutinante, pero muy baja actividad eritroaglutininante
La PHA-E no es específica de grupo sanguíneo, pero la aglutinación puede ser inhibida por ciertos oligosacáridos. La PHA-P es la forma proteica de la PHA antes de la separación y la purificación de la eritroaglutinina y la leoucoaglutinina. La PHA-M es la forma mucoproteínica. Los conjugados se preparan a partir de las correspondientes lectinas purificadas.

Preparation Note

Purified by a modification of the method of Leavitt.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of either human erythrocytes (2% in phosphate buffered saline, pH 6.8) or human leukocytes (107 per mL in saline) after 1 hr incubation at 25 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Variation in gamma interferon responses to different infecting strains of Mycobacterium tuberculosis in acid-fast bacillus smear-positive patients and household contacts in Antananarivo, Madagascar
Rakotosamimanana N, et al.
Clinical and Vaccine Immunology : CVI, 17(7), 1094-1103 (2010)
Fan Yang et al.
Gene therapy, 29(1-2), 41-54 (2021-01-13)
Adeno-Associated Virus (AAV)-based gene therapy vectors are in development for many inherited human disorders. In nonclinical studies, cellular immune responses mediated by cytotoxic T cells may target vector-transduced cells, which could impact safety and efficacy. Here, we describe the bioanalytical
Linc-MAF-4 regulates Th1/Th2 differentiation and is associated with the pathogenesis of multiple sclerosis by targeting MAF
Zhang F, et al.
Faseb Journal, 31(2), 519-525 (2016)
Carla M S Ribeiro et al.
Nature, 540(7633), 448-452 (2016-12-06)
The most prevalent route of HIV-1 infection is across mucosal tissues after sexual contact. Langerhans cells (LCs) belong to the subset of dendritic cells (DCs) that line the mucosal epithelia of vagina and foreskin and have the ability to sense
K J MacLeod et al.
Hormones and behavior, 136, 105072-105072 (2021-10-11)
Reproduction is a critical part of an animal's life history, but one which incurs significant costs to survival and future reproductive potential. These physiological consequences are likely to be influenced by context - for example, if an individual is subject
Ver todos los artículos relacionados

Artículos

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico