F3685

Fibroblast Growth Factor-Basic, human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, carrier free, suitable for cell culture

• Sinónimos: FGB-b, FGF-2, HBGF-2, HBGH-2, Prostatropin, bFGF
• UNSPSC Code: 12352202
• NACRES: NA.77

Propiedades

• product name: Fibroblast Growth Factor-Basic, FGF-Basic, from human, recombinant, expressed in E. coli, carrier free
• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: <1 ng/mL Biological Activity EC₅₀
• mol wt: 16.0 kDa
• packaging: pkg of 4X25 μg; pkg of 25 μg
• storage condition: avoid repeated freeze/thaw cycles
• color: white to faint yellow cast
• solubility: water: soluble 0.025 mg, clear, colorless to faintly yellow
• UniProt accession no.: P09038
• storage temp.: −20°C
• Gene Information: human ... FGF2(2247)

Descripción

General description

Since FGF-Basic is found in a variety of organs, acts on a wide range of cell types, and has multifunctional actions, it has acquired numerous synonyms, including heparin-binding growth factor (class II or beta), eye-derived growth factor I, cartilage-derived growth factor, and astroglial growth factor II⁵. Purified bovine and human FGF-basic differ by 3 amino acids in sequence³, and are biologically and immunologically cross-reactive.

Citation

1. Gospodarowicz, D., Localization of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth. Nature, 249, 123-127 (1974).
2. Gospodarowicz, D. et al., Structural characterization and biological functions of fibroblast growth factor. Endo. Rev., 8, 95-114 (1987).
3. Esch, F. et al., Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc. Natl. Acad. Sci. USA, 82, 6507-6511 (1985).
4. Neufeld, G., and Gospodarowicz, D., Basic and acidic fibroblast growth factors interact with the same cell surface receptors. J. Biol. Chem., 261, 5631-5637 (1986).
5. Lobb, R.R. et al., Purification of heparin-binding growth factors. Anal. Biochem., 154, 1-14 (1986).
6. Abraham, J.A., Human basic fibroblast growth factor: nucleotide sequence and genomic organization. EMBO J., 5, 2523- 2528 (1986).

Información de seguridad

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable