A2058

Seroalbúmina bovina

lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

• Sinónimos: Albúmina bovina, Albúmina sérica bovina, BSA
• Número de CAS: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC Code: 12352202
• NACRES: NA.27

Propiedades

• biological source: bovine
• product line: BioReagent
• assay: ≥95% (agarose gel electrophoresis)
• form: lyophilized powder
• mol wt: ~66 kDa
• purified by: chromatography
• packaging: poly bottle of
• origin: New Zealand origin
• technique(s): cell culture | mammalian: suitable
• impurities: IgG free; ≤0.05% IgG; ≤1 EU/mg endotoxin
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt accession no.: P02769
• storage temp.: 2-8°C
• Gene Information: bovine ... ALB(280717)

Descripción

General description

Bovine serum albumin (BSA) is a non-glycosylated protein belonging to the serum albumins family. It is a globular protein with α-helix structure and 17-disulfide bonds. BSA has a molecular weight of 66 kDa consisting three domains and two subdomains under each. BSA shows structural similarity with human serum albumin.

Application

BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide

BSA is widely used as a blocking agent in immunoassays and immunodetection procedures, and also as a carrier protein for dilution of antibodies. Thus it is of concern to be aware of potential trace endogenous content of globulins / IgG in BSA, because of concerns that such trace globulins / IgG may serve as antigens for secondary antibodies in immunodetection work. This product undergoes testing for low IgG content / status as essentially immunoglobulin-free.

Select the right albumin for your application with the Albumin Selection Guide.

Bovine Serum Albumin has been used to treat polyomavirus BK to serve as a control in testing intravenous immunoglobulins preparations.

Biochem/physiol Actions

Se sospecha que ciertos epítopos conformacionales y de secuencia primaria son alérgenos en las alergias humanas a la leche y a la carne.

Bovine Serum Albumin (BSA) acts as a carrier protein for drugs, hormones and fatty acids. It is an essential component of cell culture medium. BSA serves as a blocking agent in enzyme linked immunosorbent assay (ELISA) and prevents non-specific binding of antigens and antibodies to the microtiter plates. Due to its high solubility, low cost and purity and interaction with surfactant, it is useful in pharmaceutical and cosmetic industry.

Features and Benefits

• Tested for use in cell culture
• Low endotoxin
• Essentially IgG-free (immunoglobulin free)

Preparation Note

La seroalbúmina puede denominarse también Fracción V. Esta convención de nomenclatura procede del método original de fraccionamiento de las proteínas séricas de Cohn en el que se utiliza precipitación en etanol frío. Utilizando dicho método, la seroalbúmina se encontró en la fracción quinta del etanol. Desde entonces, algunos han venido utilizando la expresión «Fracción V» para describir la albúmina sérica, al margen del método de preparación. Otros han utilizado este término para describir la seroalbúmina purificada por métodos de fraccionamiento en etanol que se han modificado mucho desde que se describió el método Cohn original. Sigma-Aldrich fabrica y distribuye seroalbúminas purificadas mediante una variedad de métodos primarios, entre ellos el verdadero método de fraccionamiento de Cohn, los métodos de fraccionamiento en etanol modificados, el fraccionamiento térmico y la cromatografía. Etapas de purificación añadidas pueden ser la cristalización o la filtración en carbón activado.

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)