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12103C

Sigma-Aldrich

Suero fetal bovino

USA origin, suitable for cell culture

Sinónimos:

FBS, FCS, Suero, sueros

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About This Item

MDL number:
MFCD00132239
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine fetus

Quality Level

500

product line

FBS Premier

composition

hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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General description

El FBS sigue siendo un complemento popular para los medios porque aporta una amplia gama de funciones al cultivo celular. El FBS aporta nutrientes, factores de crecimiento y de unión y protege a las células del daño oxidativo y la apoptosis mediante mecanismos que son difíciles de reproducir en los sistemas de medios sin suero (SFM).

Application

El suero fetal bovino se utilizó como suplemento en diferentes tipos de cultivo celular, como los siguientes:
  • células PC12 cultivadas en el medio de Eagle modificado de Dulbecco enriquecido con suero fetal bovino junto con otros componentes.
  • Las células HeLA se mantuvieron en el medio Roswell Park Memorial Institute (RPMI) 1640 que contenía suero fetal bovino junto con otros componentes.
  • Cultivo celular BaF3 (las células se mantuvieron en medio RPMI 1640 enriquecido con suero fetal bovino junto con otros componentes).
  • Cultivos celulares primarios del hipocampo (las células se mantuvieron en el medio esencial mínimo de Eagle y se complementaron con suero bovino fetal termoinactivado junto con otros componentes).
  • Se cultivaron células de HeLA, Cos1 y Cos7 en medio Eagle modificado de Dulbecco (DMEM) con suero bovino fetal junto con otros componentes.
El suero bovino fetal (FBS) es el suero más común utilizado para enriquecer los medios de cultivo celular debido a su elevado contenido nutricional. Aunque es relativamente bajo en proteínas, el FBS promueve y mantiene con eficacia el crecimiento de células de mamíferos e insectos.

Este producto es para uso posterior en la fabricación. ESTE PRODUCTO NO ESTÁ DESTINADO A USO HUMANO NI TERAPÉUTICO.

Analysis Note

  • Analizada la presencia de endotoxinas y hemoglobina
  • Se ha estudiado la presencia de bacterias, virus y micoplasmas
  • Filtrado triple con membrana de 0,1 micra bajo condiciones asépticas

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Joseph E Rabinowitz et al.
Journal of virology, 76(2), 791-801 (2001-12-26)
The serotypes of adeno-associated virus (AAV) have the potential to become important resources for clinical gene therapy. In an effort to compare the role of serotype-specific virion shells on vector transduction, we cloned each of the serotype capsid coding domains
Julie L Webb et al.
The Journal of biological chemistry, 278(27), 25009-25013 (2003-04-30)
Parkinson's disease (PD) is characterized by the loss of dopaminergic neurons in the substantia nigra and the formation of aggregates (Lewy bodies) in neurons. alpha-Synuclein is the major protein in Lewy bodies and rare mutations in alpha-synuclein cause early-onset PD.
R J Mark et al.
Journal of neurochemistry, 68(1), 255-264 (1997-01-01)
Peroxidation of membrane lipids results in release of the aldehyde 4-hydroxynonenal (HNE), which is known to conjugate to specific amino acids of proteins and may alter their function. Because accumulating data indicate that free radicals mediate injury and death of
Tadepally Lakshmikanth et al.
Cell reports, 32(3), 107923-107923 (2020-07-23)
The human immune system varies extensively between individuals, but variation within individuals over time has not been well characterized. Systems-level analyses allow for simultaneous quantification of many interacting immune system components and the inference of global regulatory principles. Here, we
D B Bregman et al.
Proceedings of the National Academy of Sciences of the United States of America, 93(21), 11586-11590 (1996-10-15)
Damage to actively transcribed DNA is preferentially repaired by the transcription-coupled repair (TCR) system. TCR requires RNA polymerase II (Pol II), but the mechanism by which repair enzymes preferentially recognize and repair DNA lesions on Pol II-transcribed genes is incompletely
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Protocolos

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Freezing and Thawing FBS and Other Sera

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

Contenido relacionado

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Cell Culture Workflow

Our broad range of the most trusted tools for cell culture includes stringently sourced and tested FBS, established media formulations, and sterile labware. Cutting-edge techniques using stem cells and 3D matrices are enabled by organoids, hydrogels, culture scaffolds, and bioinks for 3D bioprinting.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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