GPT-RO

Glutamate-Pyruvate Transaminase (GPT)

from pig heart

• Sinónimos: alanine aminotransferase
• Comisión internacional de enzimas: 2.6.1.2 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Propiedades

• biological source: pig heart
• Quality Level: 100
• form: suspension
• specific activity: ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
• packaging: pkg of 1 mL (10105589001 [10 mg]); pkg of 2.5 mL (10737127001 [25 mg])
• manufacturer/tradename: Roche
• optimum pH: ~8.0
• shipped in: wet ice
• storage temp.: 2-8°C

Descripción

General description

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Application

Synthesis of unnatural L-amino acids from α-keto acids.

Quality

Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT

Unit Definition

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available