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Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) Alkaline Phosphatase conjugate

1 mg/mL, Chemicon®

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

ELISA: suitable
western blot: suitable

shipped in

wet ice

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to alkaline phosphatase and stabilized in buffer.

Specificity

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

ELISA: 1:3,000 (1,2)

Immunohistochemistry/immunocytochemistry: 1:200-1:500 (3,4)

Western Blot, chromogenic: 1:200- 1:500 (1,2)

Western Blot, chemiluminescent: 1:2,000-1:5,000 (1)

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Mouse IgG Antibody, (H+L) Alkaline Phosphatase conjugate is validated for use in ELISA, WB for the detection of Mouse IgG.

Physical form

ImmunoAffinity Purified

Storage and Stability

The undiluted antibody is stable at 2-80C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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