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  • Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils.

Contact-dependent inhibition of HIV-1 replication in ex vivo human tonsil cultures by polymorphonuclear neutrophils.

Cell reports. Medicine (2021-07-02)
Tatjana Reif, Gerhard Dyckhoff, Ralph Hohenberger, Carl-Christian Kolbe, Henning Gruell, Florian Klein, Eicke Latz, Bettina Stolp, Oliver T Fackler
ABSTRACT

Polymorphonuclear neutrophils (PMNs), the most abundant white blood cells, are recruited rapidly to sites of infection to exert potent anti-microbial activity. Information regarding their role in infection with human immunodeficiency virus (HIV) is limited. Here we report that addition of PMNs to HIV-infected cultures of human tonsil tissue or peripheral blood mononuclear cells causes immediate and long-lasting suppression of HIV-1 spread and virus-induced depletion of CD4 T cells. This inhibition of HIV-1 spread strictly requires PMN contact with infected cells and is not mediated by soluble factors. 2-Photon (2PM) imaging visualized contacts of PMNs with HIV-1-infected CD4 T cells in tonsil tissue that do not result in lysis or uptake of infected cells. The anti-HIV activity of PMNs also does not involve degranulation, formation of neutrophil extracellular traps, or integrin-dependent cell communication. These results reveal that PMNs efficiently blunt HIV-1 replication in primary target cells and tissue by an unconventional mechanism.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
C5a Receptor Antagonist, W-54011, The C5a Receptor Antagonist, W-54011, also referenced under CAS 405098-33-1, controls the biological activity of C5a Receptor.
Sigma-Aldrich
Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder