Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) found in various mammalian tissues, especially endothelial cells. tPA is secreted as a single-chain precursor, which is cleaved to a two-chain form by plasmin. It is supplied in the single-chain form with a molecular weight of approximately 70 kDa. The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa, primarily containing serine amino acid residue. Tissue plasminogen activator (tPA) is a protein primarily composed of the elements found in amino acids: carbon (C), hydrogen (H), oxygen (O), nitrogen (N), and sulfur (S).
T0831
Tissue plasminogen activator human
Synonym(s):
tPA
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About This Item
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.
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form
lyophilized powder
Quality Level
specific activity
≥300 KU/mg protein
mol wt
~70 kDa
impurities
HIV, HCV, and HBsAg, tested negative
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... PLAT(5327)
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General description
Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) and is found in a wide variety of mammalian tissues, especially endothelial cells. tPA is secreted as a single chain precursor, which is cleaved to a two-chain form by plasmin.[1] Supplied in the single chain form with a molecular weight of ~70 kDa.[2] The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa majorly containing serine amino acid residue. It is predominantly expressed in endothelial cells.[3][4][5]
Application
Tissue plasminogen activator has been used in a study to assess treatment risks of intracranial hemorrhage among patients with acute ischemic stroke.[6] Tissue plasminogen activator has also been used in a study to investigate the successful treatment of early thrombosis of HeartWare left ventricular assist devices with intraventricular thrombolytics.[7] Human tissue plasminogen activator has been used to study the effect of tyrosinase-related protein 2 (TRP-2) expression on melanin production in sheep.[8]
Biochem/physiol Actions
tPA is a potent activator of fibrinolysis and converts plasminogen into plasmin and also initiates cell signaling.[9][3] The plasminogen activation system may also be associated with degradation of amyloid fibrils.[10] It is also known to mediate inflammatory and extracellular matrix remodeling processes and is predicted to participate in disease progression of intracranial aneurysm indicating brain vascular malformations. Upregulation of the gene is observed in aortic aneurysms.[3]
Physical form
Lyophilized powder, from 100 mM PBS (pH 7.4), 3.5 mg/mL L-arginine, and 0.001% Tween® 80
Preparation Note
Reconstitute with dH2O to a final concentration of 1 mg/mL (e.g. 100 μg should be reconstituted in 100 μL).
Legal Information
TWEEN is a registered trademark of Croda International PLC
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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What is the elemental composition of the tissue plasminogen activator?
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What are the storage conditions and length for the reconstituted product?
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Following reconstitution, aliquot and freeze at -20°C to -70°C. Stock solutions are stable for up to 6 months. Avoid repeated freeze/thaw cycles. For additional stability a protease-free HSA or BSA may be added at 1% to the stock solution.
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