SAB4200084

Monoclonal Anti-AGO1 antibody produced in rat

~1 mg/mL, clone 4B8, purified immunoglobulin

• Synonym(s): Anti-Argonaute-1, Anti-Eukaryotic Translation Initiation Factor 2c, subunit 1, Anti-GERP95, Anti-Golgi Endoplasmic Reticulum protein 95 kDa
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 4B8, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~95 kDa
• species reactivity: human, canine, mouse, monkey, bovine
• concentration: ~1 mg/mL
• technique(s): immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using HEK-293T cell extracts
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EIF2C1(26523) ; mouse ... Eif2c1(236511)

Description

General description

Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1−4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.

Immunogen

recombinant human AGO1 fusion protein.

Application

• Monoclonal Anti-AGO1 antibody produced in rat has been used in:
• western blotting
• immunoprecipitation
• chromatin immunoprecipitation(CHIP) assay
• RNA immunoprecipitation
• RNA-nChIP experiments

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Biochem/physiol Actions

The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable