GE11-0033-99

His GraviTrap^™

Cytiva 11-0033-99

• Synonym(s): His GraviTrap Column
• UNSPSC Code: 12161501
• NACRES: NA.32

Properties

• packaging: pkg of 10 ea
• manufacturer/tradename: Cytiva 11-0033-99
• bed volume: 1 mL
• column I.D.: 15 mm
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacity: ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• suitability: suitable for bioprocess medium

Description

General description

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

Features and Benefits

• For fast and simple purification of histidine-tagged* proteins.
• Convenient, prepacked gravity-flow column.
• Allows direct purification of both clarified and unclarified cell lysates.
• High binding capacity; approximately 40 mg of pure protein per column.
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS09
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids