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Merck

d-Ribose as a Contributor to Glycated Haemoglobin.

EBioMedicine (2017-10-17)
Xixi Chen, Tao Su, Yao Chen, Yingge He, Ying Liu, Yong Xu, Yan Wei, Juan Li, Rongqiao He
ABSTRACT

Glycated haemoglobin (HbA1c) is the most important marker of hyperglycaemia in diabetes mellitus. We show that d-ribose reacts with haemoglobin, thus yielding HbA1c. Using mass spectrometry, we detected glycation of haemoglobin with d-ribose produces 10 carboxylmethyllysines (CMLs). The first-order rate constant of fructosamine formation for d-ribose was approximately 60 times higher than that for d-glucose at the initial stage. Zucker Diabetic Fatty (ZDF) rat, a common model for type 2 diabetes mellitus (T2DM), had high levels of d-ribose and HbA1c, accompanied by a decrease of transketolase (TK) in the liver. The administration of benfotiamine, an activator of TK, significantly decreased d-ribose followed by a decline in HbA1c. In clinical investigation, T2DM patients with high HbA1c had a high level of urine d-ribose, though the level of their urine d-glucose was low. That is, d-ribose contributes to HbA1c, which prompts future studies to further explore whether d-ribose plays a role in the pathophysiological mechanism of T2DM.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
4-(3-Methyl-5-oxo-2-pyrazolin-1-yl)benzoic acid, ≥98%
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S-Benzoylthiamine O-monophosphate
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Anti-β-Actin antibody, Mouse monoclonal, clone AC-15, purified from hybridoma cell culture
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Hemoglobin human, lyophilized powder