MTOXRTSUP

RPTEC Tox Supplement

sterile-filtered, suitable for cell culture, add to 500 ML of basal medium

• UNSPSC Code: 12352207
• NACRES: NA.81

Properties

• Quality Level: 300
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Sigma has developed a variety of specialized media which are intended to provide optimal performance when utilized with specific cell lines. This product consists of 6 mL of RPTEC Tox media supplement which is added to basal media and other components listed below to produce 500 mL of media needed for optimal culture conditions of Sigma′s Human Renal Proximal Tubule Epithelial Cells (RPTEC) when used for Kidney Toxicology studies. The final RPTEC Tox media was developed specifically for use with Sigma′s human RPTEC control SA7K Clone cells and various knockouts (KO).

Preparation Note

Prepare Tox Medium by supplementing 500 mL of MEMα (M4526) with 6.25 mL of L-glutamine (G7513), 6 mL RPTEC Tox Supplement (MTOXRTSUP) and 0.3 mL of Gentamicin solution (G1397) and 0.03 mL of Amphotericin B solution (A2942). Tox Medium can be stored at 2–8 °C for up to 1 month. This medium is formulated for use with a 5% CO₂ in air atmosphere.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable