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Key Documents

I4758

Sigma-Aldrich

Iminodiacetic acid Agarose

saline suspension

Synonym(s):

IDA Resin

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About This Item

MDL number:
UNSPSC Code:
23151817
PubChem Substance ID:
NACRES:
NA.56

form

saline suspension

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix activation

epoxy

matrix attachment

amino

matrix spacer

12 atoms

capacity

≥15 μmol/mL binding capacity (CuSO4)

storage temp.

2-8°C

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Application

Iminodiacetic acid-agarose is used in affinity chromatography, protein chromatography and as a chelating resin.

Physical form

Suspension in 0.5 M NaCl containing preservative

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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S S Lam et al.
Biochemical and biophysical research communications, 253(1), 135-142 (1999-01-06)
Two ribosome inactivating proteins designated alpha- and beta-pisavins were isolated from seeds of the garden pea Pisum sativum var. arvense Poir with a procedure involving affinity chromatography on Affi-gel Blue gel, immobilized metal ion affinity chromatography on Iminodiacetic acid-agarose, cation
Eva Zatloukalová et al.
Journal of separation science, 29(8), 1082-1087 (2006-07-13)
A method for the immobilization of antibodies to inert matrix represents an important factor that affects results of immunoaffinity chromatography. Binding antibodies to immobilized metal ions is an example of oriented immobilization that avoids a random coupling of a protein.
S Ghosh et al.
Gene, 176(1-2), 249-255 (1996-10-17)
Vectors have been constructed for the general purpose of expressing foreign proteins in E. coli. These vectors allow the production in high yield of either native proteins or of fusion proteins which contain, at their amino terminus, the peptide Met

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