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Key Documents

HTS000MC1

Sigma-Aldrich

ChemiSCREEN Chem-1 Membrane Preparation, Null Control

Chem-1 membrane preparation for Negative control in radioligand binding Assays.

Synonym(s):

Chem-1 Membrane Prep, ChemiSCREEN Null Control, Membrane Preparation

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32161000
NACRES:
NA.41

biological source

rat

Quality Level

recombinant

expressed in Chem-1 cells

manufacturer/tradename

ChemiScreen
Chemicon®

technique(s)

radioligand binding assay (RLBA): suitable

shipped in

dry ice

General description

Chem-1 cells are an adherent cell line endogenously overexpressing Galpha15 that is used as a host for Chemicon′s ChemiScreen GPCR Membrane Preparations. Chemicon′s ChemiScreen Wild-Type Chem-1 Membrane Preparations are ideal controls for validation of radioligand binding assays employing ChemiScreen GPCR Membrane Preparations in the Chem-1 host cell line.
None

Application

Chem-1 membrane preparation for Negative control in radioligand binding Assays.
Negative control in radioligand binding assay

Biochem/physiol Actions

GPCR Class: N/A
Protein Target: Null Control
Target Sub-Family: Control

Physical form

Membranes were adjusted to 0.5 mg protein/ml in 50 mM Tris pH 7.4, 10% glycerol and 1% BSA, rapidly frozen, and stored at -80°C. Use at the same protein concentration as the ChemiScreen GPCR Membrane Preparations of interest.

Storage and Stability

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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