1.50435
SeQuant™ ZIC®-HILIC 200Å (5 µm) HPLC Columns
L × I.D. 20 mm x 2.1 mm Guard Column
Synonym(s):
Hydrophilic Interaction Liquid Chromatography Guard Column
About This Item
Recommended Products
product name
SeQuant™ ZIC-HILIC™ Guard 20 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
Agency
suitable for USP L114
product line
SeQuant™
feature
endcapped: no
parameter
550 bar pressure
70 °C max. temp.
technique(s)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
mass spectrometry (MS): suitable (High)
column L × I.D.
2 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
5 μm
pore size
200 Å pore size
operating pH
1.5-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
Related Categories
General description
SeQuant™ ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility.
The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.
Thanks to their favorable retention and robust performance, SeQuant™ HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant™ ZIC®-HILIC for analysis of melamine and related substances. SeQuant™ HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.
Learn more about the Supelco HPLC Column portfolio and Method development:
Brochure: HPLC and UHPLC Column Selection Guide
Brochure: A Practical Guide to HPLC - Method Development Guide
Application
- Development and validation of LC-MS/MS methods to measure tobramycin and lincomycin: Demonstrates the application of SeQuant ZIC-HILIC Guard columns in a pharmacokinetic research study, focusing on the high-precision measurement of pharmaceutical compounds in biological matrices. This method is essential for ensuring accurate dosage and monitoring therapeutic levels, crucial for clinical trials and medical research (Pandey S et al., 2020).
Analysis Note
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