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Merck
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Key Documents

1.13301

Millipore

Bactident Aminopeptidase

suitable for detection, pkg of 50 strips

Synonym(s):

Test on L-alanine aminopeptidase

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About This Item

UNSPSC Code:
41116130
NACRES:
NA.24

feature

ready-to-use

Quality Level

packaging

pkg of 50 strips

manufacturer/tradename

Bactident

technique(s)

microbe id | specific enzyme detection: suitable

antibiotic activity spectrum

Gram-negative bacteria

application(s)

detection
environmental
food and beverages

storage temp.

2-8°C

suitability

Aeromonas spp.
Bacillus spp.
Salmonella spp.

General description

BACTIDENT Aminopeptidase test strips are diagnostic test strips used in microbiology to detect the activity of aminopeptidase enzymes in bacterial strains. Aminopeptidases are a group of enzymes that cleave amino acids from the amino terminus of polypeptides and proteins. The test strips contain L-alanine-4-nitroanilide as a substrate, which is split into 4-nitroaniline and the amino acid L-alanine in the presence of the enzyme alanine aminotransferase. The L-alanine aminopeptidase enzyme is localized in the cell envelope of bacteria, and the development of clear yellow coloration of 4-nitroaniline indicates the presence of the enzyme.

Application

BACTIDENT Aminopeptidase test strips are useful for bioburden testing in the industry. The aminopeptidase test is a reliable method for determining the Gram behavior and differentiating Gram-positive from Gram-negative bacteria. These test strips are used for the detection of L-alanine aminopeptidase-producing microorganisms. The test is particularly useful in identifying Enterobacteriaceae and in differentiating between species of clinically important bacteria such as Streptococcus and Enterococcus.

Linkage

Replaces: 1.13301.0002

Analysis Note

Colour change to yellow
- Salmonella typhimurium ATCC 14028 (WDCM 00031):
+
- Aeromonas hydrophila ATCC 7966 (WDCM 00063): +
- Proteus rettgeri ATCC 29944: +
- Yersinia enterocolitica ATCC 9610 (WDCM 00038): +
- Bacillus cereus ATCC 11778 (WDCM 00001): -
- Staphylococcus aureus ATCC 25923 (WDCM 00034): -
- Enterococcus faecalis ATCC 11700: -

Legal Information

BACTIDENT is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

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