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  • Electronic readout enzyme-linked immunosorbent assay with organic field-effect transistors as a preeclampsia prognostic.

Electronic readout enzyme-linked immunosorbent assay with organic field-effect transistors as a preeclampsia prognostic.

Advanced materials (Deerfield Beach, Fla.) (2014-07-23)
Mallory L Hammock, Oren Knopfmacher, Tse Nga Ng, Jeffrey B-H Tok, Zhenan Bao
PMID25047764
ABSTRACT

Organic field-effect transistor (OFET) sensors can meet the need for portable and real-time diagnostics. An electronicreadout enzyme-linked immunosorbent assay using OFETs for the detection of a panel of three biomarkers in complex media to create a pre-eclampsia prognostic is demonstrated, along with biodetection utilizing a fully inkjet-printed and flexible OFET to underscore our ability to produce disposable devices.

MATERIALS
Product Number
Brand
Product Description
49163
Sigma-Aldrich
Glucose solution, BioUltra, for molecular biology, ~20% in H2O
158968
Sigma-Aldrich
α-D-Glucose, anhydrous, 96%
G1951
Sigma-Aldrich
D-Gluconic acid solution, 49-53 wt. % in H2O
G8644
Sigma-Aldrich
D-(+)-Glucose solution, 100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture
G8769
Sigma-Aldrich
D-(+)-Glucose solution, 45% in H2O, sterile-filtered, BioXtra, suitable for cell culture
G3285
Supelco
D-(+)-Glucose solution, 1 mg/mL in 0.1% benzoic acid, standard for enzymatic assay kits GAGO20, GAHK20, STA20, analytical standard