A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

A peptide from human semenogelin I self-assembles into a pH-responsive hydrogel.

Soft matter (2014-11-20)

B Frohm, J E DeNizio, D S M Lee, L Gentile, U Olsson, J Malm, K S Akerfeldt, S Linse

ABSTRACT

The peptide GSFSIQYTYHV derived from human semenogelin I forms a transparent hydrogel through spontaneous self-assembly in water at neutral pH. Linear rheology measurements demonstrate that the gel shows a dominating elastic response over a large frequency interval. CD, fluorescence and FTIR spectroscopy and cryo-TEM studies imply long fibrillar aggregates of extended β-sheet. Dynamic light scattering data indicate that the fibril lengths are of the order of micrometers. Time-dependent thioflavin T fluorescence shows that fibril formation by GSFSIQYTYHV is a nucleated reaction. The peptide may serve as basis for development of smart biomaterials of low immunogenicity suitable for biomedical applications, including drug delivery and wound healing.

MATERIALS

Product Number

Brand

Product Description

75746

Sigma-Aldrich

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