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Merck
  • Growth rate influences reductive biodegradation of the organophosphorus pesticide demeton by Corynebacterium glutamicum.

Growth rate influences reductive biodegradation of the organophosphorus pesticide demeton by Corynebacterium glutamicum.

Biodegradation (2001-10-06)
L Girbal, J L Rols, N D Lindley
ABSTRACT

The organophosphorous pesticide, demeton-S-methyl was transformed by Corynebacterium glutamicum in co-metabolism with more readily degradable substrates. Glucose, acetate and fructose were tested as growth substrates, and the highest demeton-S-methyl biotransformation average rate (0.78 mg l(-1) h(-1)) and maximum instantaneous rate (1.4 mg l(-1) h(-1)) were achieved on fructose. This higher efficiency seems to be linked to the atypical behavior of C. glutamicum grown on fructose, characterized by a prolonged period of accelerating growth instead of a constant growth rate observed on glucose or acetate. More precisely, for growth rates in the 0.1-0.4 h(-1) range, a direct coupling between the specific demeton-S-methyl consumption rate and the growth rate was demonstrated on fructose during batch-, steady state continuous- or continuous cultures with a controlled transient growth rate (accelerostat technology). The demeton-S-methyl biotransformation was more favoured during an acceleration phase of the growth rate.

MATERIALS
Product Number
Brand
Product Description

Supelco
Disulfoton, PESTANAL®, analytical standard