SRP2070
LRH-1 human
recombinant, expressed in insect cells, ≥70% (SDS-PAGE)
Synonym(s):
B1F, B1F2, CPF, FTF, FTZ-F1, FTZ-F1beta, LRH-1, LRH1, hB1F-2
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.26
Recommended Products
biological source
human
recombinant
expressed in insect cells
Assay
≥70% (SDS-PAGE)
form
frozen liquid
mol wt
~62.6 kDa
packaging
pkg of 5 μg
concentration
50 μg/mL
color
clear colorless
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... NR5A2(2494)
Biochem/physiol Actions
LRH-1 or HB1F for human B1-binding factor belongs to the fushi tarazu factor-1 (FTZ-F1) subfamily of orphan nuclear receptors and is closely related to steroidogenic factor-1. LRH1 contains a DNA-binding domain with 2 zinc finger motifs, an FTZ-F1 box, and a ligand-binding domain. Cholesterol 7-α-hydroxylase is the first and rate-limiting enzyme in a pathway through which cholesterol is metabolized to bile acids. Elevated promoter-specific repressor protein (SHP) inactivates LRH1 by forming a heterodimeric complex that leads to promoter-specific repression of both CYP7A1 and SHP. These results revealed an elaborate autoregulatory cascade mediated by nuclear receptors for the maintenance of hepatic cholesterol catabolism. LRH1 specifically binds and activates viral hepatitis B enhancer II, an essential element for the liver-specific regulation of hepatitis B virus gene expression. CPF is a key regulator of human CYP7A gene expression in the liver. SHP1 represses expression of CYP7A1 by inhibiting the activity of LRH1 which regulates CYP7A1 expression positively. This bile acid-activated regulatory cascade provides a molecular basis for the coordinate suppression of CYP7A1 and other genes involved in bile acid biosynthesis.
Physical form
Clear and colorless frozen liquid solution
Preparation Note
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Lu, T. T., et al.
Molecular and Cellular Biology, 6, 507-515 (2000)
M Li et al.
The Journal of biological chemistry, 273(44), 29022-29031 (1998-10-24)
Enhancer II (ENII) of hepatitis B virus (HBV) is one of the essential cis-elements for the transcriptional regulation of HBV gene expression. Its function is highly liver-specific, suggesting that liver-enriched transcriptional factors play critical roles in regulating the activity of
Goodwin, B., et al.
Molecular and Cellular Biology, 25, 517-526 (2000)
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