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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
11B-10G, monoclonal
mol wt
calculated mol wt 196.36 kDa
observed mol wt ~190 kDa
purified by
using protein G
species reactivity
rat
packaging
antibody small pack of 100 μL
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... Unc13a(64829)
General description
Protein unc-13 homolog A (UniProt: Q62768; also known as Munc13-1) is encoded by the Unc13a (also known as Unc13h1) gene (Gene ID: 64829) in rat. Munc13-1 is a large multifunctional peripheral membrane protein that is essential for synaptic vesicle fusion and neurotransmitter release. It is expressed in brain, with highest levels observed in the olfactory bulb, striatum, cerebral cortex, hippocampus, and cerebellum. Some expression has also been reported in pancreatic islet cells. Munc13-1 is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and also participates in the activity-dependent refilling of readily releasable vesicle pool. It contains a variable N-terminal region that contains a C2A domain and a calmodulin-binding region (CaMb) and a conserved C-terminal region that contains the C1, C2B, MUN and C2C domains. The C2A domain can form either a homodimer or a heterodimer with RIMs and provides a switch that controls neurotransmitter release and couples exocytosis to diverse forms of Rab3- and RIM-dependent presynaptic plasticity. The CaMb region is reported to mediate Ca2+-dependent short-term plasticity. The C1 domain mediates diacylglycerol and phorbol ester-dependent potentiation of release and the C2B domain acts as a Ca2+- and PIP2-dependent modulator of short-term plasticity. The MUN domain is highly elongated and is essential for opening syntaxin-1. In the presynaptic terminal, Munc13-1 is shown to form multiple and discrete supramolecular self-assemblies that serve as independent vesicular release sites by recruiting syntaxin-1. At the synaptic contacts in the active zone, Munc13-1 is essential for the formation of the syntaxin-1/Munc18-1 complex. This supramolecular assembly provides open syntaxin-1 molecules for use in the formation of active SNARE complexes and thereby specifies the site for synaptic vesicle docking, priming, and release. Munc3-1 is shown to be essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. (Ref.: Quade, B., et al. (2019). eLife 8; e42806; Sakamoto, H., et al. (2018). Nat. Neurosci. 21(1); 41-49).
Specificity
Clone 11B-10G is a mouse monoclonal antibody that detects Munc-13-1 protein in rat. It targets an epitope within 70 amino acids from the N-terminal half.
Immunogen
His-tagged recombinant fragment corresponding to the first 320 amino acids from rat Protein unc-13 homolog A (Munc13-1).
Application
Quality Control Testing
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Munc13-1 in Rat brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Munc13-1 in Immunocytochemistry applications (Sakamoto, H., et al. (2018). Nat Neurosci.;21(1):41-49).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Rat brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Munc13-1 in Rat brain tissue lysate.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Munc13-1 in Immunocytochemistry applications (Sakamoto, H., et al. (2018). Nat Neurosci.;21(1):41-49).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Munc13-1, clone 11B-10G, Cat. No. MABN2642, is a mouse monoclonal antibody that detects Protein unc-13 homolog A and is tested for use in Immunocytochemistry and Western Blotting.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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