Skip to Content
Merck
  • The reduction in electroporation voltages by the addition of a surfactant to planar lipid bilayers.

The reduction in electroporation voltages by the addition of a surfactant to planar lipid bilayers.

Biophysical journal (1998-07-24)
G C Troiano, L Tung, V Sharma, K J Stebe
ABSTRACT

The effects of a nonionic surfactant, octaethyleneglycol mono n-dodecyl ether (C12E8), on the electroporation of planar bilayer lipid membranes made of the synthetic lipid 1-pamitoyl 2-oleoyl phosphatidylcholine (POPC), was studied. High-amplitude ( approximately 100-450 mV) rectangular voltage pulses were used to electroporate the bilayers, followed by a prolonged, low-amplitude ( approximately 65 mV) voltage clamp to monitor the ensuing changes in transmembrane conductance. The electroporation thresholds of the membranes were found for rectangular voltage pulses of given durations. The strength-duration relationship was determined over a range from 10 micros to 10 s. The addition of C12E8 at concentrations of 0.1, 1, and 10 microM to the bath surrounding the membranes decreased the electroporation threshold monotonically with concentration for all durations (p < 0.0001). The decrease from control values ranged from 10% to 40%, depending on surfactant concentration and pulse duration. For a 10-micros pulse, the transmembrane conductance 150 micros after electroporation (G150) increased monotonically with the surfactant concentration (p = 0.007 for 10 microM C12E8). These findings suggest that C12E8 incorporates into POPC bilayers, allowing electroporation at lower intensities and/or shorter durations, and demonstrate that surfactants can be used to manipulate the electroporation threshold of lipid bilayers.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Octaethylene glycol monododecyl ether, ≥98% (GC)
Sigma-Aldrich
Octaethylene glycol monododecyl ether, BioXtra, ≥98.0% (GC)