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Merck

Specific interaction among some enzymes and sodium dodecyl sulfate.

Life sciences (1982-08-02)
M T Vincenzini, F Favilli, C Treves, P Vanni
ABSTRACT

The effect of 1-butanesulfonic acid sodium salt and sodium dodecyl sulfate on the activity of highly purified and crystalline enzymes with marked differences in structure and function has been studied. The enzymes were: alcohol dehydrogenase; lactate dehydrogenase; malate dehydrogenase; isocitrate dehydrogenase; glucose-6-phosphate dehydrogenase; lipase; alkaline phosphatase. While 1-butanesulfonic acid sodium salt, at the studied concentrations, resulted generally inactive, sodium dedecyl sulfate showed a selective inhibitory effect, always under the critical micellar concentration. A kinetic analysis of the inhibitory action was also carried out.

MATERIALS
Product Number
Brand
Product Description

Supelco
Sodium 1-butanesulfonate, suitable for ion pair chromatography, LiChropur, ≥99.0% (T)
Sigma-Aldrich
Sodium 1-butanesulfonate, anionic detergent
Sigma-Aldrich
Sodium 1-butanesulfonate, 98%