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  • Glycyl betaine is effective in slowing down the irreversible denaturation of a detergent-solubilized membrane protein, sarcoplasmic reticulum Ca2+-ATPase (SERCA1a).

Glycyl betaine is effective in slowing down the irreversible denaturation of a detergent-solubilized membrane protein, sarcoplasmic reticulum Ca2+-ATPase (SERCA1a).

Biochemical and biophysical research communications (2009-12-17)
Cédric Montigny, Bertrand Arnou, Philippe Champeil
ABSTRACT

Many membrane proteins become labile when they are solubilized by detergent. Here we show that the presence of high concentrations of glycyl betaine stabilizes one of these proteins, the sarcoplasmic reticulum Ca(2+)-ATPase (SERCA1a), solubilized with nonionic detergents like n-dodecyl beta-d-maltopyranoside (DDM) or octaethylene glycol monododecyl ether (C(12)E(8)) which are commonly used for its purification or crystallization. Betaine at high concentrations might become useful as a stabilizing agent for detergent-solubilized membrane proteins, for instance during purification procedures or during the long periods of time required for crystallogenesis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Octaethylene glycol monododecyl ether, ≥98% (GC)
Sigma-Aldrich
Octaethylene glycol monododecyl ether, BioXtra, ≥98.0% (GC)