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Key Documents

G005862

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 15 cm x 4.6 mm I.D., 2.7 μm particles (53931-U)
mobile phase: methanol
flow rate: 1.0 mL/min
pressure: 1653 psi (114 bar)
column temp.: 30 °C
detector: UV, 280 nm
injection: 5 μL
sample: 0.4 mg/mL tocopherol acetate, alpha-tocopherol and K1, 0.04 mg/mL D2 and D3, all in ethanol

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

Analysis was conducted using standard solution, detection via UV.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Ergocalciferol (D2), analytical standard
    Cholecalciferol (D3), analytical standard
    DL-alpha-Tocopherol acetate, analytical standard
    (±)-α-Tocopherol, analytical standard
    Phylloquinone (K1), analytical standard

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