M3024
Minimum Essential Medium Eagle
with Earle′s salts and non-essential amino acids, without L-glutamine, phenol red and sodium bicarbonate, Modified, powder, suitable for cell culture
Synonym(s):
EMEM, MEM
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
Earle’s salts (5% CO2): yes
L-glutamine: no
HEPES: no
NaHCO3: no
sodium pyruvate: no
phenol red: no
shipped in
ambient
storage temp.
2-8°C
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General description
Minimum Essential Medium Eagle contains 19 amino acids. The essential amino acids and the non-essential amino acids: L-ala, L-asn, L-asp, L-glu, L-gly, L-pro, and L-ser. This medium is preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium. It is suitable for a wide variety of cells grown in monolayers.
Application
Minimum Essential Medium Eagle has been used:
- to culture endometrial adenocarcinoma Ishikawa cells for bisphenol studies
- to culture MCF-7 cells for experimental treatments with hormone
- to maintain zebrafish pineal glands for action spectrum analysis
Features and Benefits
Contains high concentrations of amino acids so the medium resembles the protein composition of cultured mammalian cells
Quantity
Formulated to contain 9.3 grams of powder per liter of medium.
Reconstitution
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
also commonly purchased with this product
Product No.
Description
Pricing
supplement
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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