K1128

α-Ketoglutaric acid

BioReagent, suitable for cell culture, suitable for insect cell culture

• Synonym(s): 2-Oxoglutaric acid, 2-Oxopentanedioic acid
• Linear Formula: HOOCCH₂CH₂COCOOH
• CAS Number: 328-50-7
• Molecular Weight: 146.10
• Beilstein: 1705689
• EC Number: 206-330-3
• MDL number: MFCD00004165
• UNSPSC Code: 12352204
• PubChem Substance ID: 24896202
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥98.5% NaOH basis (titration)
• form: powder or crystals
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable
• solubility: water: 100 mg/mL, clear to slightly hazy, colorless to almost colorless
• storage temp.: 2-8°C
• SMILES string: OC(=O)CCC(=O)C(O)=O
• InChI: 1S/C5H6O5/c6-3(5(9)10)1-2-4(7)8/h1-2H2,(H,7,8)(H,9,10)
• InChI key: KPGXRSRHYNQIFN-UHFFFAOYSA-N

Description

General description

Alpha-ketoglutarate (αKG), a vital intermediate in the tricarboxylic acid (TCA)/Krebs cycle, is located between succinyl-CoA and isocitrate.

Application

α-Ketoglutaric acid has been used:

• as a supplement in minimum essential medium α (α-MEM) to culture primary mesenchymal stromal/stem cells (MSCs) to study its effects on age-related osteoporosis
• for chemical treatments to compare the treatment effects of several compounds on lifespan between manual and automated lifespan machine (ALM) platforms
• as a printed chemotaxis ligand to screen chemotaxis receptors with high-throughput technologies and verify the interactions with surface plasmon resonance (SPR)

Biochem/physiol Actions

Alpha-ketoglutarate (αKG), a nitrogen scavenger, is an important source of glutamate and glutamine. It controls the synthesis of ATP and decreases the generation of equivalent (NAD+/NADH) in the tricarboxylic acid (TCA) cycle. It promotes the synthesis of protein and prevents protein degradation in muscles. This immune nutrient factor participates in the general immune and collagen metabolism. In aged rodents, αKG accelerates the healing of bone defects. It may possess potential anti-aging effects. This Krebs cycle molecule can be used as a supplement in insect and mammalian cell cultures.

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H318
• Precautionary Statements: P280 - P305 + P351 + P338 + P310
• Hazard Classifications: Eye Dam. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves