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22178

Sigma-Aldrich

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

form

powder

specific activity

~0.8 U/mg

color

off-white

storage temp.

2-8°C

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General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Application

Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Unit Definition

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A novel system for trigger-controlled drug release from polymer capsules
Fluri David A, et al.
Journal of Controlled Release : Official Journal of the Controlled Release Society, 131(3), 211-219 (2008)
G. Okada
Methods in Enzymology, 160, 259-259 (1988)
P. Toussaint et al.
Entropie, 23 (136), 8-8 (1987)
María Dolores Ayala et al.
Animals : an open access journal from MDPI, 10(12) (2020-12-05)
A 90-d feeding trial was conducted in which five groups of gilthead seabream (11.96 g initial body weight) were fed with a microalgae-free diet (control group, C) or four diets containing the microalgae Nannochloropsis gaditana at two inclusion levels (2.5%
Helena Fernandes et al.
Bioresource technology, 281, 277-285 (2019-03-03)
The macroalgae aquaculture industry has grown up in the last years, and new applications for macroalgae should be considered. In this work, sequential biological treatments as solid-state fermentation (SSF) by Aspergillus ibericus and enzymatic hydrolysis (EH) were applied to washed

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