00185
Acetamide Nutrient Broth
suitable for microbiology, NutriSelect® Basic
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About This Item
Recommended Products
sterility
non-sterile
Quality Level
product line
BioChemika
form
powder
shelf life
limited shelf life, expiry date on the label
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbe id | utilization test: suitable
final pH
7.0±0.2 (25 °C)
application(s)
bioburden testing
environmental
food and beverages
water monitoring
microbiology
suitability
selective by defined nutrients for Pseudomonas spp.
Application
Synthetic medium to detect microbial utilization of acetamide. Corresponds to the suggestions of the "Deutsches Institut für Normung (DIN)" and the test procedures set out in §35 LMBG for the examination of water for Pseudomonas aeruginosa.
Components
Composition
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
Preparation Note
Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Other Notes
Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Carc. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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H.H. Guenich et al.
Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, 252, 230-230 (1982)
R H Schubert et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 233(3), 342-346 (1975-11-01)
The comparative examination of the acetamide-decompostition and of the acetamide-utilisation as sole source of carbon demonstrates that various groups of the Pseudomonas species with the same kind of reaction have to be distinguished. The acetamide-decomposition-test according to BUHLMANN et al.
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