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HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions)

HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53916-U

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

standard

Product No.
Description
Pricing

47845

Sorbic acid

analytical standard

06185

Benzoic acid

Standard for quantitative NMR, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)

flow rate

0.4 mL/min

pressure

138 bar (2000 psi) column, 248 bar (3600 psi) total

column temp.

55 °C

detector

UV, 215 nm

injection

1 μL

sample

50 μg/mL in mobile phase

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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